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Kapillärelektrofores
CE, CGE och CEC
The integrated PrinCE 700 Series
 
The PrinCE 700 series is a powerful integrated Capillary Electro Chromatography system with a very
sensitive Diode Array Detector (DAD). It is fully automated, flexible, modular and suitable for almost
any application.
Thanks to the original modularity of the PrinCE system, it is still easy to couple to any external CE
detector, or to MS.
The integrated systems can handle up to 32 samples without
interference of the operator, while still maintaining 10 buffer vials.
Any combination of six segments (sample/buffer type) can be used. The segments can be exchanged
easily by the operator without needing any tools. The possible segments are 5 positions for buffers or
8 positions for samples.
Under precisely controlled circumstances it is possible to use the integrated PrinCE 700 series for
fraction collection, but this requires a lot of accuracy and precision of the operator. Because of the
minute sample amounts-so typical in CE- dilution of the fractions cannot be avoided.
Some features of the integrated PrinCE 700 series are:
* Short effective capillary length: 7.5 cm, with total minimum capillary length of 33 cm
* Hardware selectable inlet and outlet for short or long effective capillary length
* Complete temperature control of capillary
* Capillary cassette
* Three types of DAD possible: 256, 512 or 1024 diodes
* Measurements in UV, VIS and NIR
* 3D analysis software, including: high resolution spectra, spectra library search, peak purity calculations,
peak identification
* Low limit of detection
* Easy to couple to MS and other CE detector (like Conductivity, Fluorescense...)
New feature:
Additional external fibre cell: offers the possibility to select between the standard internal fibre cell
which goes with the PrinCE 700 series or select an optional remote fibre cell. This remote fibre cell
is fully automated selectable and increases the flexibility of the PrinCE system and is ideal in combination
with a Mass Spectrometer (MS) because the sample can be measured very closely to the used MS.
The cell with fibres can be connected to the detector part of the PrinCE 700 series using a set of panel
fit SMA connectors. The additional external fibre cell is not standard available but optional as a factory
set modification only.
Specifications of the Prince 700 Series
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Injection modes
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Hydrodynamic injection uses patended Dynamic Compression Injection (DCI) to
generate and apply a range of positive and negative pressures with controlled
ramping (-180 to 250 mbar, 1 mbar resolution). Electrokinetic injection features
controlled ramping in voltage or current mode (max. 30 kV)
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Pressure features
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On inlet, outlet or both.
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Flushing pressure
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0 to +2000 mbar by DCI device, up to 10 bar by external pressure
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Voltage range
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-30 kV to +30 kV
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Current range
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-200 µA to + 200 µA
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Ramping
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Programmable voltage or current ramping
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Polarity reversal
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Programmable pre-run and during the run
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Autosampler/
fraction collector
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30/48 position carousel sample and buffer vials randomly accessible from
both capillary ends. Variable vial sizes of samples and buffers available,
including Eppendorf micro centrifuge tubes.
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Buffer volume
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Maximal 4 ml
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Sample volume
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Minimal 10 µl, maximal 4 ml
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Temperature control
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Complete temperature control of the capillary: 5°C below ambient till 60°C
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Capillary
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Effective length: 7.5 cm, minimal total length 33 cm
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Sophisticated features
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Current or voltage controlled method step durations (for on-line pre-concentration).
Positive or negative pressure during electrophoresis. Outlet-and injections of sample
or reagents.
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Power requirement
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115/230 V, 50/60 Hz, 300 VA
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Footprint
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61 x 45 x 48 cm (w x h x d)
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Weight
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45 kg
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Accessories
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A start-up kit of sample and buffer vials
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Integrated DAD
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Three different types of High sensitive Diode Array Detectors available:
256 diodes with a range from 190 nm up to 720 nm
512 diodes with a range from 190 nm up to 610 nm
1024 diodes with a range from 190 nm up to 1010 nm
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External Detector
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Allows the use of any CE detector including MS, Fluorescence and Conductivity
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Software
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Our DAx-3D analysis software.
This software is specially made for our integrated PrinCE 700 series
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PrinCE Autosampler and Fraction Collector 
The modular system design allows the PrinCE to be configured to individual
needs. Options include a 4-position sample tray or a random access carrousel with accommodation for 30-48 sample/buffer vials and Peltier control of temperature to protect samples from evaporation and degeneration.
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