Introducing the cHiPLC™-nanoflex system:
making nanoLC/MS easier and more reproducible

The new cHiPLC-nanoflex, in combination with the nanoLC-Ultra system and any mass spectrometer/nanospray source, delivers superior sensitivity, excellent column-to-column reproducibility, and ease of operation.
A "docking station" for up to three microfluidic chips, the system's flexible design and built-in 10-port nano valve allow for easy switching between direct injection, trap-loading and dual column with direct injection experiments.

Eksigent has introduced the ChipLC-nanoflex System, which aims to deliver reproducibility to proteomics research.

The system is a docking station for up to three microfluidic chips that contain nanoLC or trap columns.
Eksigent's Eksport microconnector system ensures reliable and consistent fluidic connections to facilitate column changes.
Chips can be interchanged in seconds, even by inexperienced users, with minimal dead volume to compromise resolution.
The microfabrication process used for both the trap chips and analytical column chips delivers exceptional column-to-column reproducibility, claims the company. Quality separations and reproducibility are critical to the success of nanoLC-MS-based biomarker validation studies.
In these applications, where peptides are measured by multiple reaction monitoring (MRM), the improved column-to-column reproducibility delivered by the ChipLC-nanoflex helps in obtaining consistent data from large numbers of samples.
The ChipLC-nanoflex can be added to any of Eksigent's nanoLC systems. It can also be used with Applied Biosystems Tempo nano LC systems.

ChipLC-nanoflex and Eksigent's nanoLC systems can be integrated with mass spectrometers including Thermo, Applied Biosystems/MDS SCIEX and Bruker Daltonics.
A built-in 10-port nano valve allows for switching between different types of experiments such as direct injection and trap loading.

Eksigent has introduced the ExpressHT-Ultra HPLC system, providing short cycle times and high throughput for LC/MS studies of pharmacokinetics and drug metabolism.

This system is the latest offering in the company's line of products for small molecule analysis. With cycle times as short as 60 seconds, the ExpressHT-Ultra can run six 96-well sample plates overnight.
Running at a maximum pressure of 10,000psi, the system enables the use of small particle stationary phases to deliver narrow peaks to the mass spectrometer.
Employing columns with diameters of 1mm or smaller, the ExpressHT-Ultra uses up to 95 per cent less solvent per analysis than traditional HPLC systems.
'Drug discovery and development researchers need a higher throughput LC solution to process ever-increasing numbers of samples,' said David Neyer, product manager for Eksigent.
'Our technology delivers fast separations at low flow rates to meet the growing demands of LC/MS applications.' Eksigent's microfluidic flow control (MFC) technology generates accurate and reproducible flow rates from nanolitres per minute to hundreds of microlitres per minute.

Eksigent Offers New MALDI Spotting Capability with Single-Point Control from NanoLC

"This new capability enables customers to use our powerful NanoLC systems in MALDI applications," explained Remco van Soest, product manager for Eksigent.  "Our NanoLC is already the leading platform for proteomics applications, and many customers have been using our LC with a third-party spotter.  Now we can provide them with a solution that is fully integrated and optimized for our NanoLC."

The Ekspot spotter/fraction collector deposits fractions eluted from the NanoLC onto a MALDI plate, while a built-in pump automatically adds the matrix.  High positioning accuracy ensures consistent spotting with maximum resolution.  The high-capacity system stores up to sixteen MALDI-targets for high-throughput applications.

By eliminating flow splitting, Eksigent's proprietary microfluidic technology improves reproducibility and sensitivity and eliminates solvent waste.  The NanoLC's precise microscale flow control runs HPLC gradients at rates as low as 20 nL per minute.

Eksigent serves the life science, drug discovery and pharmaceutical industries, expanding customer possibilities with low-flow fluid delivery solutions.  From precise separation of precious samples in proteomics research to fast and efficient analytical chromatography to unique medical devices that improve the way drugs are delivered, Eksigent brings novel solutions to customer challenges.  The company combines proprietary microfluidic pumping technology with creative approaches that help customers improve productivity and expand their research and market opportunities.  Eksigent serves customers throughout the world, including Europe, Asia and Australia, through a growing sales and service network.

The Eksigent NanoLC-2D system is the only nanoscale HPLC system that combines the following features in one instrument:
• Two independent high pressure binary gradient pumps. Flow rate 1-20 µl/min for gradient pump 1, and 20-1000 nl/min for gradient pump 2. Allows for on-line 2D analysis, and parallel separations.
• Splitless (direct) pumps with flow monitoring and feedback to guarantee flow rate accuracy and reproducibility independent of backpressure and viscosity.
• Gradient delay volume < 65 nl.
• Gradient retention time reproducibility < 0.5% RSD .
• Allows for peak parking by rapidly changing the flow rate down to 5 nl/min in less than 4 seconds. No additional valves and/or pumps are required for peak parking. Peak parking extends Mass Spectrometry acquisition time for increased sensitivity or complex samples.
• Compact footprint of 18”x 22” (w x d).
• Software control fully integrated with Xcalibur (Thermo), Analyst (Applied Biosystems) and Hystar (Bruker) Mass Spectrometry software.

The optional NanoLC-AS1 autosampler has the following unique features:
• Programmable injection volume from 200 nl to 10 µl with 10 µl loop.
• Dual needle design: steel needle for puncturing of septum and fused silica needle for aspiration of sample. This design allows for aspiration of sample from volumes as small as 2 µl using conical vials.
• Precision of < 0.5% RSD for partial loop injections.
• Can be used with autosampler vials or microtiter plates.
• Bio-inert titanium injection valve.
• Sample cooling and heating (4-40 °C).
• Software control fully integrated with Xcalibur (Thermo), Analyst (Applied Biosystems) and Hystar (Bruker) Mass Spectrometry software.
• Fits on top of a NanoLC 2D system : 11” x 16” (w x d).

go microflow for ultra-fast LC/MS
Why use ultra-high pressure when you can use the ExpressLC-100 and consume less solvent, save money, cut analysis time in half and realize high resolution and sensitivity? Read about the results GSK achieved using the ExpressLC-100 for open-access LC/MS.
learn more

microfluidic flow control applied to fast LC/MS of Pharmaceutical Small Molecules GlaxoSmithKline presented a poster at the recent ASMS conference which explores the use of Eksigent's ExpressLC system combined with mass spec for rapid open-access LC/MS. The poster examines system performance, throughput, and robustness in a walk-up, open-access environment. To cover a broad range of analytes from multiple medicinal chemists, GSK uses a "universal" gradient method running a full gradient in one minute. Benefits of the ExpressLC system for LC/MS cited by GSK include: The ExpressLC system is a viable alternative to ultra-high pressure systems in terms of resolution and sensitivity The system is robust, durable and convenient The ExpressLC system cuts current analysis time in half Solvent consumption is reduced by >99% Estimated savings on solvent purchase/disposal is $2,000 per 10,000 samples Universal method is applicable to most pharmaceutical small molecules

Related Links: Download a copy of the poster

Uvi Light XT Dimension The Uvi Light XT Dimension is a complete and powerful spectrophotometer for routines or advanced applications. Uvi Light XT Dimension is equipped with high performance optics and software with built-in hard drive and floppy disks to allow local storage and transfer of files. To further simplify the operation, the Uvi Light XT Dimension utilized a standard computer keyboard, mouse and display. Advanced measurement modes in Abs/%T, concentration, kinetics, spectrum scanning and multi-wavelengths are just a few of this feature packed instrument. With curves and methods storage on internal hard drive, you have the capacity to store 1000 methods and 250 spectrums. The Uvi Light XT Dimension offers a reduced investment cost, ergonomic design and an uncompromising feature set combined with ease of use.

PRIM Light The PRIM Light is a compact spectrophotometer for simple measurements in absorbance, transmission or concentration using 1 factor or 1 standard. PRIM Light is a user-friendly spectrophotometer, especially designed for very simple operations even for first-time users. Up to 50 tests with parameters can be stored in permanent memory and recalled at anytime. For precise and repeatable results, Prim includes a built-in didymium calibration filter and upon each start-up, it performs an auto-calibration over the full spectrum. Printouts include both date and time in order to conform to GLP regulation.

PASTEL-UV Portable Analyzer for Water Quality Traditional measurement methods are not adequate to cope with the problems of water resources and with environmental attacks. SECOMAM, has developed an alternative technique based on the analysis of UV absorption spectra of the samples. This technique brings about a considerable gain in term both of cost and time. The PASTEL-UV is able to measure 6 parameters simultaneously and thus permits rapid characterization of the effluent. Due to its unique design, each sample can be analyzed in approximately one minute which allows to carry out a large number of measurements and to react in real time in cases of accidental pollution or of a process incident. Simplicity is the key to our design which allows even non-chemists to easily operate this instrument. Since there are no costly reagents or consumables to worry about, your cost of analysis is as low as your sample cost!

Download: Application Shampoo Washing powder Instruments used LINEX CombiPAL OPTIC 3 PHASER Shimadzu 2010 Complex matrices are encountered in numerous application areas of gas chromatography. They occur for example in environmental analysis, food characterisation or in the analysis of home and personal care products. For such difficult matrices the technique DMI (Difficult Matrix Introduction) is a powerful analytical tool. In the present contribution the DMI technique is applied for screening cosmetic products, such as lipsticks, crèmes, washing powders and shampoos. Almost no sample preparation for shampoo and washing powder: GC-MS analysis of shampoo or washing powder is generally difficult because the compounds of interest are present at low levels in a complex water and oil containing emulsion. Using DMI, the matrix influence of shampoo or washing powder can be almost eliminated. A small aliquot of the shampoo or some washing powder is put into a small glass vial placed into the liner which is automatically inserted into the injector. The injector is then heated to 120°C just high enough to transfer the compounds of interest from the sample onto the chromatographic column. Only the vaporized compounds are transferred from the injector onto the GC-column where they are refocused at the low starting temperature of the GC program. Because the non-volatile matrix remains in the vial, no injector liner and column contamination occurs. To automate the analysis a liner exchanger (LINEX) is used. OPTIC 3 news: ATAS GL has further developed and improved their standard OPTIC 3 injector. All new OPTIC 3 instruments have now an improved ramp rate of 30°C/sec. Starting from the 1st of April a new control software is available with both pressure and flow control. On top of this there is an injector body available that can adapt ¼"OD x 3.5" liners. visit our web site

Portable Versatile Zeeman Mercury Analyser

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Analytical Ultracentrifugation: Techniques and Methods

Analytical Ultracentrifugation has become an increasingly important technique for monitoring the size and shape of biological macromolecules.

Analytical Ultracentrifugation: Techniques and Methods contains contributions from experts in the field, bringing together the multitude of developments that have taken place in instrumentation and analysis over the past decade into a single volume.

The book covers the latest methods in analysis along with an extensive introduction for the novice user. Analysis methods in both sedimentation velocity and sedimentation equilibrium are discussed at length. Protein, Protein/DNA, membrane proteins and polymer systems are also explored, along with software developments and non-ideality.

Chapter 14 is an Introduction to Differential Sedimentation and is based on the CPS Disc Centrifuge DC24000.

The book is ideal for biochemists, biophysicists and polymer chemists with an interest in analytical ultracentrifugation. The ISBN number is 0-85404-547-3.

You can order the book either from info@crelab.se or the Royal Society of Chemistry in the UK.